Problem description: if run the runfile of cuda, will appear the error of gzip: stdin: unexpected end of file

Solution: the installer on the official website of nvidia sometimes have problems, and it is found that the md5 proof test value does not match with the official website, so it is speculated that the download is incomplete.

Solution: bring your own available cuda installation files

Problem description: relion3 cannot import tiff raw data for subsequent processing.

Solution: it may be a software compilation problem for relion3. The system environment does not have libtiff library when the software is compiled.

Solution: yum install libtiff-devel (centos system )Install libtiff, then recompile relion3.

Problem description: there is error for display of Manual pick. And the error content is ’std::out_of_range’，what(): basic_string::substr

Solution idea: this problem is basically the software bug, and we need to find out the cause of triggering the bug.

Solution: after importing a sheet of data, use Display directly to report the same error. Check that the data folder in the working directory of relion has been processed by soft link (linked from other paths). By contrast, it is found that directly copying an image into the data folder can open normally.

Problem description: when relion3.0 operates Gctf and makes particle_local_ctf_refine, the defocus UV value remains unchanged after successfully calculating more than a dozen graphs.

Solution: after testing, it is found that there would be problem when this version of the Gctf. （Gctf-v1.06_sm_30_cu8.0_x86_64and Gctf-v1.18_sm_30_cu8.0_x86_64）operate local_ctf, and this may be due to system, GPU, cuda, and program version compatibility.

Solution: after testing, change the Gctf version to Gctf_v1.18_b2_sm60_cu8.0 to solve the problem.

Problem description: when relion3.0 operates Gctf, the program estimated time is 0 all the time.

Solution: check the corresponding relation of graphics cards with cuda and Gctf versions.

Solution: download the appropriate software version.